Linked immunosorbent munhu akaidza: kushanda uye zvimwe

Linked immunosorbent munhu akaidza - rabhoritari kudzidza, iyo inobva kuziva zvivako "antigen-antibody" chaiwo enzymatic Labels kuzvibata, nokuchinja ruvara kubva substrate. Chinangwa chikuru ongororo iyi - Kutsunga kuvapo siyana zvibereko enzymatic siyana pabvunzo ivhu.

Nokuti kuziva miedzo maantigen uye antibodies heterogeneous enzayemu immunoassay rinoshandiswa, sezvo kwayo muitiro zviri nyore kuti kuparadzanisa zvakamisa hurongwa zvakaitwa uye kunonoka vamwe vari yakasimba chokutambudzika.

Zvinokosha kucherechedza zvinotevera zvakanakira ichi rabhoritari yokudzidza:

• yakakwirira utetsa, izvo zvinogona kuona kunyange vashoma mamorekuru pakati zvinhu;

• yokuita ongororo iyi vanogona kushandisa risingamboshandisi nhamba yomuedzo zvinhu;

• mukana refu kuchengeta zvose zvakamisa hurongwa kuti anodiwa kuti ongororo;

• Easy kuti aite;

• Kuwanikwa richishanda uye ziso zvabuda;

• immunosorbent munhu akaidza iri zvoga padanho rimwe nemapinduriro;

• vashoma shoma mutengo Kits inoshandiswa kuongorora chirwere.

zvakwakanakira izvi kushandisa chaizvo IFA muzvinhu siyana mishonga, kusanganisira, nokuti chirwere ose wanda.

The inonyanya nzira nokuda immobilizing antibodies uye maantigen chiri adsorption maitiro, kana nokwenzvimbo mamorekuru dzakabatanidzwa yakasimba danho (nokuda Ionic kana hydrophobic mukukurukurirana kana inotungamirwa rehydrogen kusungwa).

Ndinofanira kutaura kuti enzayemu immunoassay inoshandiswa nyaya mbiri:

• kuziva maantigen pamusoro utachiona kutapukirwa siyana;

• kuona pamberi antibodies kusvika antigen pamusoro pathological tusingaoneki (zvinofanira kucherechedzwa kuti Elisa anogona kuziva chero dzokubereka nadzo chirwere).

Special polystyrene ndiro rakashandiswa kuita serodiagnosis ichi. Rine 96 mumwena panhivi adsorbed antigen. Pamusoro kupinda ari pahwendefa vakaidza sero antigen antibodies vari zvakabatanidzwa zvakakodzera. Zvakazoitwa kuwedzera munhu akanzi antibody kuti pamatsime immunoglobulins dzevanhu kunosvika chaiyo kuita. Munyaya inotevera kurapwa serum chromogen (dhayi) dzinosiyana coloring substrate (mu pamberi porutivi zvivako "antigen-antibody"). Zvinokosha kucherechedza kuti ruvara kusimba ndiyo Proportional kuti uwandu antibodies zvakananga.

Elisa Analysis apedza kuyera Manyiminya arambe achirema nemvura iri mumatsime. Saka kushandisa mano uye stereotyped mapatani kuti zvinobatsira kuverenga antibody wevasungwa. Zvinofanira kurangarirwa kuti ose bvunzo hurongwa kuitisa ongororo iyi ine tsika neezvitendero zviratidzo (izvi kazhinji nezvaro zvabuda, ayo akapa).

Uchishandisa Elisa rokuongorora njovhera, nemavhairasi kuzvimba chiropa uye HIV, uyewo anowanikwa mumuviri womunhu cytomegalovirus, Chlamydia, herpes (mhando dzakasiyana). Ongororo iyi kunobatsirawo kuona pachiyero mahormone kuziva kenza zvezvinangwa.

Where kuti kuedzwa muMoscow? Nhasi, kune yakawanda unyanzvi mumarabhoritari, iyo kupa zvakawanda serological nemiedzo. Kuti vawane nzira tapota ona chiremba yekwataigara kiriniki (private kana nyanzvi, iwe dveryaete). Pamwe pakuongororwa ndiendewo kwachiremba. Uye kuteerera mashoko ake pamusoro kudiwa kurapwa.