Ploskireva svikiro (Bacto-Hagari F): tsananguro. Medical zvidukuduku

Ploskireva svikiro (hereinafter vachinzi muchiri Bacto Agari F) ndiye inotonhorera svikiro kuti kurima zvimwe tupukanana tusingaoneki, kunyanya Shigella uye Salmonella. Seinokonzera wake kushandiswa utachiona zvinhu: weti, bile, mutsvina yadzo.

nyaya

Nikolay Ivanovich Ploskirev - Soviet microbiologist uye Kukudzwa chiremba. Akaberekerwa muna 1869 uye akafa muna 1948, vakati akashanda akawanda hwake guta rake - Tomsk.

Muna 1888, iye, musayendisiti yemberi, pasina kupedza mugiredhi rechitanhatu kuchikoro, akabatana muchiuto. akakwanisa kupedza dzidzo chete muna 1892, ipapo vakapfuura bvunzo kuti kubvuma kuti Imperial Tomsk University, akadzidza paChikoro rorusununguko Medicine. Muna 1898 akagamuchira musoro chiremba.

Ploskirev vaiitawo Russian-Japanese War, uye pashure pacho aishanda Tomsk muchipatara. Muna 1910 akagadzwa kuti pambiru musoro Tomsk dermatological uye venereal chipatara, uye kwemakore makumi matatu kwave musoro wayo zvachose. Chete musayendisiti aiva mumwe havaoni kuti njovhera kiriniki iri Tomsk.

Nikolay Ivanovich Ploskirev akanyora nhevedzano mabasa hwakatsaurirwa pakurwisa venereal zvirwere.

mamiriro

Wednesday Ploskireva - Naizvozvo zvinhu nekuda kurima intestinal utachiona uye, vanofanira ane mhando zvakawanda zvinovaka muviri. It anogadzirwa yakaoma chimiro.

Zvikuru yakakura inorwara kwayo zvachose kurema ndiwo pancreatic hydrolyzate sprat (10.4 g / l). Zvishoma zvishoma zvawanikwa hydrocitrate dibasic yavaibvisa (8.5 g / l). Uyewo pane yakaoma inotonhorera muto uye mukaka shuga (8.62 uye 7,3 g / l).

Chechipiri rokuremekedza Ploskireva unosvikirwa - J. Bacto-Hagari iri murwiyo zviri aripo munguva yakawanda Hagari, 6,94 g / l. Seriovatistokislogo anhydrous yavaibvisa tigutsikane yakaenzana 5.1 g / l. Pane kuvapo anhydrous disodium phosphate - 2,1 g / L, yavaibvisa munyu pamusoro cholic asidhi vanenge 3,46 g / L uye pasoda nedota - 2.4 d

Pasati giremu zviri mukati zviratidzi - kwazvakarerekera tsvuku (0.05) uye Pazvinhu mitema (0.0002). The ayodhini kugutsikana - 0,13.

kunyoresa

The kudzidza nokukanganisika dzinotsanagura mabhakitiriya anobatsira chaizvo pakuita nechirwere kwezvirwere anokonzera anaerobic zvinenge. Species mumhuri iyi - mazana. Ndivo anenge akafanana Morphology netsika zvivako, uye hwakavimbika zvikuru nzira yokusiyanisa kubva mumwe - kudzidza nemakemikari omuviri.

The kudzidza rinotanga nemashoko inoculation pamusoro pathological zvinhu kupinda tsika svikiro. Iye pathological differential nokuda coliform utachiona uye chikamu chacho, kuwedzera pachena Hagari, rinosanganisira chiedza uye lactose. Processing mano lactose - ane chiratidzo inokosha Kusiyanisa kuti enterobacteria. Kana izvi kuona ph vachambokanganwa vakananga acidity uye chinoratidza iri kusashanda, iyo kwaanoda munzvimbo.

Dzimwe nyika vanogona kushandisa rimwe inotonhorera zvenhau, asi mashandiro avo chiito rinoenderana vatatu rakajairika muRussia - ezvinhu ichi Endo uye Levine Ploskireva Wednesday.

Description of nzira

Kunze Ploskireva analoginen svikiro iri anonzi Macconkey Hagari. In rapera chimiro läpinäkyvä mhinduro ine chiedza blue-yellow ruvara. Mapoka kukwanisa ironge lactose, pakati Ploskireva kupa tsvuku (cranberry) ruvara. Kana utachiona havagoni kubata nayo, kana nyika iri Hauoneki kana ane simba ruvara.

Yandakapiwa Kuronga svikiro zvinosanganisira zvinhu Ploskireva FIs (Pazvinhu rwegirini, bile munyu, ayodhini), zvinonzi anenge chose kuchititadzisa kukura giremu positive kasvisto, sapamazuva rokutanga eshehiry yekudyidzana retards ari kukura, uyewo dzimwe dzose concomitant microflora.

chechipiri gumbo

Humwe kufarira ndiyo kusarudzwa nyuchi, uye seeded pamusoro chikuru chekuchengeta svikiro uye Kusiyanisa. Environment umo mune seeding vanofanira ane vakawanda substrates. Chirwere tsika vanofanira kuona enzymatic basa noruremekedzo kwavari, kuti chete svikiro mukavaisa sepombi kuitira kuti pane nzvimbo mbiri:

• mumwe umo slanting Hagari;
• ane mbiru.

Kufarira vatsvakurudzi nyuchi vakawanda bevelled mugove gobvu sitiroko uye iri shongwe - munhu anobaya. Panguva iyi, svikiro akashandisa Russell, Kligler kana Olkenitskogo. Sezvo differential chirwere kushandiswa uye Ploskireva zvakatipoteredza.

Zvidukuduku uye pathogenic Utachiona

Hagari Ploskireva chinhu chinokosha Detective zvirwere zvinokonzerwa Enterobacteriaceae. Saka, Somuenzaniso, yakura uye kusiyaniswa dzaitongwa zvigare anokonzera bacillary nemanyoka. Izvi anaerobic zvinenge kungova genus Schigella.

Kufanana wose chikamu mhuri intestinal utachiona, shigella vane chimiro tsvimbo, kukura micrometers maviri. Hadzisi capsules uye spores vane flagella - nayo dzinovabvumira kusiyaniswa kubva Salmonella, izvo, akavapindurawo, muri pafoni. Vanokura zvakanaka nyore zvikuru nhau, pane imwe adziye kupfuura ambient (35-37 ° C) uye 7,4 ph. By hunhu kukura, nyuchi regai usiyane Salmonella.

Sezvakakurukurwa munyaya apa, utachiona hunogona kuva substantially morphological kusiyana Zvisinei, siyana yekudyidzana mu nokukanganisika dzinotsanagura upenyu. Saka, kunze Shigella Nyukestl, pasina paumbwe iine acid gasi kunoitika munguva chinovira pamusoro makabhohaidhiretsi. Kusara Shigella sonnei, havagoni ichivira lactose, asi ari ozeyewa shuga. Just dzavo dzinokosha features, unogona undodziisa zvavanokwanisa kuderedza niraty kuti nitrite. nyuchi dzavo dzakanga ozeyewa pamwe urea, uyewo vari mutsika svikiro hakuna liquefaction kuti gelatin.

Collection uye gadziriro mbeu

Kuziva vaishandira nemanyoka, zvinodiwa microbiological miedzo nemadandemutande runyararo, kureva faeces murwere. Pamusoro kutambira mashoko, unofanira nokukurumidza sezvinobvira kuita nokudyara. Kana zvisingaiti, panobva chinofanira kuiswa zvisaora - phosphate buffer muvhenganiswa kana glycerol. Pana 4 ° C, vanogona inochengeterwa kupfuura zuva. Unganidzai mashoko kunofanira kuitwa achishandisa rectal regirazi chubhu akaiswa mukati mugaranhoko.

Nokuti kudzidza mumwe zvinhu yasarudzwa purulent madzihwa zvidimbu kuti kushambidzwa maviri matatu sepombi isotonic yavaibvisa chloride mhinduro.

Kushandisa pathogenic utachiona vari zvakatipoteredza Ploskireva akaita magirazi spatula. Unofanira varikwizire pamusoro chikamu chiduku ayo Hagari, ipapo Achabvambura spatula zvakatipoteredza, uye residual zvinhu iri kuzorwa kupinda Dzinodyarwa pamusoro. Kana seeding kunoitwa mune makapu shoma, zvino mumwe nomumwe wavo aifanira yokusima batch mangé itsva.

Kana muco-purulent zvidimbu ikoko kuita vakazvitsaurira pachisara hazvirevi kuti hapana kuvapo utachiona. Pakadaro zvinokosha emulsify mutsvina yadzo muna 10 ml yavaibvisa chloride (wevasungwa 0,85%) uye zvadaro kudyara rimwe kana maviri madonhwe ari svikiro Ploskireva. Non-emulsified cheya zvinogona zvadyarwa selenitovy muto. Akashandisawo, kana, pane mutsvina yadzo vari mumarutsi kana Kusuka.

Microbiological kuongorora chirwere

In padariro wokutanga microbiological zveBhaibheri kunze kudyarwa pathogenic utachiona vari makapu maviri, ipapo kutarisa sei kukura Shigella. On Wednesday Ploskireva yakabereka zvibereko, uye wechipiri - zvakatipoteredza kana Endo Levin.

Nokuda kuti paiva okuva shigella vari nemishonga inouraya mabhakitiriya, kuti svikiro akanga vaisanganisa pamwe chloramphenicol. Zvadaro kunoitika mukati mazuva incubation ari mazai chenguva duku panguva 37 ° C.

Pazuva rechipiri kuongorora akura dzaitongwa. Vari kwete differential chirwere svikiro akura Hauoneki, zvakakodzera Anosakurawo panze naRussell svikiro kana pfupi "mutsara motley." Uyezve kutsvakurudza chichiitirwa nokuda algorithm chezvinhu zvirimwa pamusoro pavo. Mapoka Shigella neChitatu Ploskireva kurai muchimiro yakajeka, Hauoneki uye diki mbiru. Ivo vari marudzi maviri:

• flattened pamwe kuenzana mativi
• woita, convex, pamwe randinenge nyoro chivheneke.

Matatu kana mana dzaitongwa zvinofanira mikroskopirovat uye aongororwe kuti mufambo. Kana zvapedzisira haaratidzi kumusoro, ivo vakanogara neChitatu Olkenitskogo chokubvisa yakachena mutsika. Pasina mamirire dzaitongwa Shigella kana kana pasina kukura iri kuchengetwa zvachose, zvakakodzera Endo Hagari kana Ploskireva zvibereko seeding kuti selenitovogo muto. Kana yokuita dzaidzorwa varipo yakakwana nhamba, akaisa vanofungidzirwa agglutination. A musanganiswa Sera sonnei uye Flexner, maitiro kunoitika pamusoro girazi.

kumwe kutsvakurudza

Pazuva retatu pane kuchinja zvakaitika ari mapoka ari svikiro Russell. Kana pane mutsika chisingaurayisi zvinoshatisa kuti lactose uye shuga inovirisa ichibudisa acid, zviri akatsaurwa uye vakaongorora, vanopedza microscopy, uyewo basa vanodyara mu "mumutsara motley," asi nguva ino kuwedzerwa. Just Akaisa agglutination maitiro, kuti serological zvitupa.

Pazuva rokupedzisira, rinogona akapedzisa pachishandiswa kuchinja "motley nhamba" (kana paine chinovira pamusoro makabhohaidhiretsi), uyewo ipfupiso agglutination zvakaitwa.

Storage uye gadziriro

Kugadzirira Hagari Ploskireva kunoitika sezvinotevera:

1. 55 d yakaoma fuma kututuma ari ity pamusoro kwakadonhera mvura.
2. Vakabika maviri matatu maminitsi kusvikira Hagari haisati chose yakarukutika.
3. Dururirai mu Petri madhishi (kusabereka optional) paiva 5-6 mm.
4. Mahwendefa akavhurika kuti hafu yeawa pa nzvimbo tembiricha (18-25 madhigirii). Shure kwenguva ino svikiro iri zvakakwana omarara uye yakaoma.

Wednesday Ploskireva photosensitive uye hygroscopic. The upfu zvinofanira kuchengetwerwa rakanamwa pasuru, ari hama mwando mukamuri vanofanira haapfuuri 60%. The optimum tembiricha kuchengetera 2 ° C 25 ° C. Storage vanofanira kutevedza mitemo iyi, zvimwe zvinogoneka kururama zvakawanikwa.