Chii kamera Goryaeva? Mitemo masero eropa ngaakudze

Today Goryaeva kamera ndechimwe chezvinhu kushandiswa mano mune murabhoritari Diagnostics. Inogona kushandiswa nhamba masero siyana ropa. Sezvo chikazozikamwa, bvunzo ropa kunokosha kwazvo nedanho nechirwere uye kurapwa kusvika ose chirwere.

Chii kamera Goryaeva?

Kwakadaro inozivikanwa uye anobatsira mudziyo yambokurukurwa uye chiremba Russian aronga N. K. Goryaevym. Goryaeva kamera, chokwadi, chinhu chaizvo girazi yemasiraidhi zvemapuranga ane nemaziso pajira riya.

Sei kushandisa kamera ichi? Zvikurukuru rinogona kukubatsira kuona nhamba masero eropa, zvikurukuru leukocytes uye erythrocytes. Sezvineiwo, achibatsirwa kwayo zvinokwanisika kuona zvibereko zveminda maikorosikopu kushandiswa. The yemaitiro nokuti izvi sezvinotevera:

• Makirogiramu = (m2 - M1) / An

Muchiitiko ichi:

• Makirogiramu - zvibereko zveminda maikorosikopu;
• m2 - nzvimbo kurudyi muganhu Goryaeva kamera sero;
• M1 - nzvimbo kumuganhu rworuboshwewo;
• uye - kukura sero rimwe chete, izvo zviri mwero uye 0,05 mm;
• N - ndiyo nhamba masero pakati akayera mukamuri miganhu.

The nemamiriro kamera

Goryaev kamera chinhu innoe sechinhu girazi yemasiraidhi (asi zvikuru mukobvu kupfuura dzose) yakakamurwa transverse grooves migove mitatu. The pakati Chikamu girazi rine grid kuti kuverengwa. Chikamu chete kamuri inoshandiswa nezvizoro coverglass - nokudaro, muchengeti iri wakasika namahwindo kamuri rirambe richibvira dzezvimwe mumativi uye kuburikidza icho yazara mura.

Kana afoot, kuti Goryaeva mukamuri rakakamurwa 225 akaenzana makuru zvakaenzana saizi - vari iri gumi pemitsara. 25 makuru dzinoungana vari mberi rakakamurwa zviduku, 16 mumwe nomumwe. Jira rimwe nerimwe divi duku mativi ari 0,05 mm.

Kugadzirira kukarukureta kuti akaumba zvinhu

Zvechokwadi, muchiitiko ichi, zvinokosha unyanzvi kuti mumarabhoritari bvunzo. Chokwadi, zvose Goryaeva mukamuri hukawanika zvinofanira kuva zvakachena uye yakaoma. Pashure paizvozvo, kavha girazi ngaakuyire kuitira kuti unogona kuona maitiro mhete yakajeka. Zvinokosha kuti kamuri haana mhepo matema, sezvo izvi zvinogona kukanganisa migumisiro kuongorora.

Sezvingatarisirwa, kuti nhamba corpuscles, reagents yakasiyana inoshandiswa nomumwe zvakasiyana-siyana emasero ropa. Somuenzaniso, kushandiswa kuverengwa erythrocytes 0,9% yavaibvisa chloride mhinduro. Ezvokunambira kunze kwomuviri waamai kusanganisa 8 Rine mamiririta munyu uye 0.02 ml ropa. Saka, rabhoritari dilutes ropa 400 nguva. Dzimwe nguva ruminant inogona zvikuru.

Kuti nhamba leukocytes, zvakafanira kuti kusanganisa 0,4 ml acetic asidhi (kutora 3% kana 5% mhinduro) uye 0.02 ml ropa.

Mushure zvokubikisa kusanganiswa nemuedzo chubhu, uchishandisa chaiyo pipetting pakuwana shoma musanganiswa uye nokungwarira yazara mukamuri kuti nhoroondo (yemanyorero, mumwe kana vaviri Madonhwe zvaizova zvakakwana).

Sei ngaaverenge nhamba masero eropa?

Tichiverenga dzvuku ropa masero ari Goryaeva mukamuri rinoitwa munzvimbo dzinoungana shanu guru, izvo zvakaenzana makumi masere duku. Kuti kusakanganisa nokuda gogonera kugovera ropa ivhu, kusarudza dzinoungana akaronga Tsanga yacho yaichekwa. Special pfungwa rwakabhadharwa kuna masero yaiva kumativi kumicheto - vanoonekwa matsvuku masero kuruboshwe uye pamusoro pamadziro; asi haufaniri kufungawo avo vari pasi uye mitsetse zvakanaka.

Kuti tizive uwandu matsvuku masero por milliliter ropa, nhamba masero nemapoka makuru mashanu kane 20 000 (kana rakasanganiswa 400 times).

Leukocytes mukamuri Goryaeva kufunga zvakasiyana. Pano zvakafanira kuti ngaaverenge nhamba zvinhu zvinenge zana guru dzinoungana. The zvichiguma nhamba yakakamurwa 1600, uye ipapo kane 4000, uye zvadaro 20 (dilution).