Recombinant erythropoietin. Recombinant yevanhu erythropoietin

Erythropoietin (EPO) - glycoprotein kemikari kuti anodzora mwero erythropoiesis muvanhu. The chipo remubatanidzwa iri synthesized kunyanya itsvo, chiduku (anenge 10 muzana) anogadzirwa chiropa. The kemikari erythropoietin zvaanopa simba weboka uye Kusiyanisa pamusoro erythroid madzitateguru. Kwevari endogenous kemikari ari plasma utano vanhu kunosiyana-siyana mukati zvakawanda uye iri inversely Proportional kuti Chizenga wevasungwa uye ukuru oxygenation kuti munyama. Basa voga uye kugadzirwa kemikari ichi kuti mushonga inobatsira chaizvo.

Chimiro uye ukoshi kemikari

The Morekuru ine amino acids kemikari. At kushayiwa endogenous erythropoietin rave unopinza kuderera padanho Chizenga uye masero matsvuku eropa, zviri kuwedzera vanonzi eritropoetindefitsitnaya kupererwa neropa. akaramba zvisingabviri nokuda kwokushayiwa zvakakodzera mishonga Kutozosvikira munguva ichangopfuura, mumwe mushonga nokururamiswa kupererwa neropa yakadaro. Nhasi, ane dhefisiti pamusoro kemikari mumuviri womunhu, vanachiremba akarayira recombinant erythropoietin. Kugadzirira akawana kubva masero mhuka umo akasuma Gene remitemo kuti munhu EPO. Recombinant erythropoietin chomunhu amino asidhi uye carbohydrate sezvamunoita ndiye akafanana kune dzinongoitika kemikari, anowedzera uwandu masero eropa matsvuku, reticulocytes, zvaanopa simba iri biosynthesis kuti Chizenga ari mumasero. The chaivo basa kunoguma zvinhu hakuna rakasiyana endogenous kemikari. Recombinant erythropoietin hazvirevi kuratidza cytotoxic migumisiro uye asingauyi kukanganisa leucopoiesis. Masayendisiti zvinoratidza kuti EPO interacts zvimwe eritropoetinchuvstvitelnymi receptors, izvo iri muchitokisi pamusoro.

A muitiro okuvanatsa recombinant kwevanhu erythropoietin

Recombinant dzevanhu EPO - mumwe kupfuura zhinji mapuroteni zvinoberekwa zhinji zvoutachiona uye nemakambani anogadzira mishonga munyika kuti zvinodhaka mishonga. The chipo remubatanidzwa iri synthesized masero ovary Chinese BoyzMikaro (Cho) ne recombinant DNA unyanzvi. Mumwe polypeptide cheni recombinant EPO rine 165 amino acids, kuti vakaverenga samolecular kurema 24.000 Hongu, uye vakachengeta samolecular kurema glycosylated mapuroteni - 30 400 Da. Branch erythropoietin pakusachena riri kuitwa Ion-Exchange uye sungano chromatography. Human recombinant EPO ane kuchena 98%.

Recombinant erythropoietin uye analogs ayo

Kuti akurudzire erythropoiesis muitiro vanachiremba kushandisa zvinodhaka zvakasiyana:

• "Aranesp";
• "Aeprin";
• "Epobiokrin";
• "Bioein";
• "Vepoks";
• "Binokrit";
• "Epokrin";
• "Gemax";
• "Epogen";
• "Eprex";
• "Epovitan";
• "Epomaks";
• "Giperkrit";
• "Eralfon";
• "Eritrea";
• "Recormon";
• "Epostim";
• "Epozino";
• "Epoetin Beta".

Usati kutsiva munhu recombinant erythropoietin analogues, vanofanira kuona chiremba.

zvairatidza

Varapi kazhinji kugadzirisa eritropoetindefitsitnymi kupererwa neropa (EDA). boka iri zvinosanganisira zvinotevera pathologies:

• kupererwa neropa pamusoro nyanyisa neoplasms;
• mangwanani kupererwa neropa kuti Prematurity nendumure (pamberi vhiki 34 gestation) aine muviri kurema 750 kusvika 1500 d;
• nephrogenic kupererwa neropa;
• Kupererwa neropa mu chisingaperi zvirwere (hepatitis C, dzinonzi rheumatoid arthritis, utachiona hweHIV, alimentary Canal zvirwere).

A pachena chepfungwa pamusoro-kushaikwa kupererwa neropa ndechokuti havana kurapwa gadziriro simbi. Kutozosvikira munguva ichangopfuura, chete abudirire kurapa ndiyo ropa. Nokuti uyu mhando yokurapa ane kuwanda nemishonga uye yakakwirira pangozi kutapurirana utachiona kubva ngozi zvinotapuriranwa (HIV, hepatitis nemavhairasi, etc.) ropa nezvipembenene, nzira iyi mushonga ano zvinenge kumboshandisa. Recombinant dzevanhu erythropoietin zvinobatsira kuvandudza upenyu varwere chirwere kuti "eritropoetindefitsitnaya kupererwa neropa." Biosynthesis uye okusuma recombinant dzevanhu EPO akazarura kwenguva itsva pakurapa kupererwa neropa eritropoetinzavisimyh.

Contraindications

Vanachiremba havana kutokurudzira kushandiswa recombinant erythropoietin (Chinyorwa chinonyevera nezvazvo) pamberi zvinotevera pathologies:

• thromboembolism;
• hypersensitivity kuti zvinodhaka;
• hazvibviri zvinobudirira anticoagulant mishonga;
• Kutamiswa chepfungwa Kurohwa kana myocardial infarction;
• kusagadzikana angina;
• kusingadzoreki neBP;
• nguva rokubereka vana uye achiyamwa;
• refractory neBP.

dosing zvaiitwa

The chipimo chemushonga uye nguva kurapwa zano makasimba tisingatauri nomumwe hwaro, zvichienderana kwokuomerwa kupererwa, mukuru ezvinhu omurwere uye yezvakaita zvokurwara. The mushonga ari dzaizopiwa parenteral kushandiswa. Initial mishonga inogona siyana ari zvinobva 50 kusvika 150 Iu / makirogiramu. The dose inofanira kuchinjwa zvichienderana murwere zera. Recombinant erythropoietin Kazhinji chinotarisirwa 3 pavhiki. Kana ovhadhozi yave kuwedzera marwadzo. Mugumisiro anova zvakawanda kana zvishoma zvinoonekwa mushure memasvondo 2-3 chikumbiro.

divi kushanda

Iwe akagadza recombinant erythropoietin kwevanhu? Mirayiridzo Use haadi haabatanidzi kukura nemishonga. Ivo:

• myalgia;
• dzungu;
• hyperthermia;
• hope;
• kurutsa;
• kurwadziwa nemusoro;
• manyoka;
• arthralgia;
• neBP;
• bhokisi marwadzo;
• thrombocytosis;
• tachycardia;
• vane chirwere cheBP dambudziko;
• nokugwinha;
• steatosis;
• kukwidza huwandu AST, alt muropa;
• erythrocyte utachiona Sero aplasia;
• eczema;
• angioedema;
• ganda upundu uye kuvaviwa;
• asthenia;
• mikoko, akatsvuka nokupisa pa nzvimbo chourongwa;
• hyperkalemia;
• rutapudzo mapuroteni wevasungwa munguva yeplasma ferritin;
• hyperphosphatemia.

Erythropoiesis muviri mumitambo

Munguva ichangopfuura, anowanzoshandiswa zvinodhaka "Epovitan" (recombinant dzevanhu erythropoietin). The chipo chishandiso rinowanzoshandiswa mumitambo (mitambo, mukusimbisa muviri, kushambira, biathlon). Akati gadziriro zvaanopa simba iri biosynthesis masero matsvuku eropa, izvo zvinozoita kunotungamirira nokuwedzera zviri okisijeni chete chikwata vhoriyamu ropa uye, naizvozvo, kuti kuwedzera ropa okisijeni vanokwanisa uye O ₂ hurukuro kune mapazi uye munyama. mashandiro ichi kuita kunowedzera Kurovedza kutsungirira mumhanyi. A ndozvinoitawo ari kuchengetwa kana kudzidzisa mumhanyi ari Midlands, apo kushaya O ₂ mudenga kunokonzera kukura hypoxia, izvo zvinozoita zvaanopa simba iri biosynthesis pamusoro endogenous erythropoietin. EPO gadziriro ikashandiswa pamwe jekiseni, kukura kemikari (GH, somatotropin) uye stanazolol.

Kunwisa, kushandiswa kusingadzoreki kuti "Erythropoietin" mushonga mumitambo zvinogona kukonzera clots ropa kuti kazhinji kunotungamirira kurufu. Wanikidza recombinant EPO rakanyatsojeka zvakaoma, nokuti chakaita zvokugadzirwa yeimwe Dendere iri akafanana wacho vasiri analoginen, saka biosoedinenie ichi vachiri zvisiri pamutemo kushandiswa mukuru-waivako mitambo sezvo doping.